lipid staining/permanent

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From:Gayle Callis <>
Date:Sat, 7 Aug 1999 11:26:16 -0600

There is one way to stain and get a permanent stain for soluble lipids in
tissuse.  You postfix the tissue in osmium tetroxide, process and section.
Osmium tetroxide is not fun to work with, however, requires a good fume hood,
plus gloves, and should not be drain dumped (we can't, have to collect the
heavy metal!)  However lipids are black, an H&E stain can be applied, sections
dehydrated and coverslipped normally.  I knew one diagnostic lab who used this
routinely, but the choice is whether you want to handle and use osmium
tetroxide.  If interested I will dig the reference out of the J
Histotechnology, many years back!

Gayle Callis

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