lipid staining/permanent
<< Previous Message | Next Message >>
From: | Gayle Callis <uvsgc@msu.oscs.montana.edu> |
To: | histonet@pathology.swmed.edu |
Reply-To: | |
Date: | Sat, 7 Aug 1999 11:26:16 -0600 |
Content-Type: | |
There is one way to stain and get a permanent stain for soluble lipids in
tissuse. You postfix the tissue in osmium tetroxide, process and section.
Osmium tetroxide is not fun to work with, however, requires a good fume hood,
plus gloves, and should not be drain dumped (we can't, have to collect the
heavy metal!) However lipids are black, an H&E stain can be applied, sections
dehydrated and coverslipped normally. I knew one diagnostic lab who used this
routinely, but the choice is whether you want to handle and use osmium
tetroxide. If interested I will dig the reference out of the J
Histotechnology, many years back!
Gayle Callis
<< Previous Message | Next Message >>