Can someone tell me what buffer(s) are recommended for fixation of
avian brain tissues. I've been using a 10% formol-saline solution which
seems to work ok for staining with cresyl-violet. However, I would like to
use some immunocytochemical stains (e. g. calbindin) and note in the
literature that 4% paraformaldehyde in phosphate buffer is used for this
and similar stains. A precise formulation would be appreciated, or, where
to get a commerically available mix that requires addition of water.
Shipping overseas a consideration. Please send response to above address
and to reb@ravel.zoology.wisc.edu.

Rob Bleiweiss

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	Maureen Decorah, Research Animal Resources Center
	385A Enzyme Institute, 1710 University Avenue
	University of Wisconsin - Madison, Madison, WI 53705-4098
	(608) 262-0933  Fax(608) 265-2698  E-mail: decorah@rarc.wisc.edu
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