Re: [Histonet] isopropyl

From:"Alexandra Meinl"

Hello,Do you prefer isopropyl alcohol over ethanol?I'm also thinking about using isopropyl for our specimens.Isopropyl dehydrates more slowly, but how long is "more" when compared toethanol?Are there any limitations (e.g. are there stainings that can't be done onisopropyl dehydrated tissue)?Thanks,Alex2008/8/29 Rene J Buesa > It is an alcohol with three carbons, with a higher molecular weight (30%> more) and with the alcohol group in the center carbon, slightly less> miscible in water so less hydrophylic (dehydrates more slowly), but> regardless of all this, if it works better for you, use it.> René J.>>

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