Most times this effect is caused by uneven dewaxing of the slides.You should have a very strict protocol for exchanging the dewaxing reagents.
René J.

--- On Fri, 8/8/08, richard shook  wrote:

From: richard shook 
Subject: [Histonet] uneven ihc staining
To: histonet@lists.utsouthwestern.edu
Date: Friday, August 8, 2008, 8:50 AM

Im looking for help we are having uneven staining on some (not all) ihc slides
we have looked into several different possoble reasons with no luck ,this
happens a couple times every week ao so any suggestion would be great 


      

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