F4/80 is used in mouse tissue, it does not work in rat tissue.

Liz 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
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-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Caroline Bass
Sent: Thursday, August 07, 2008 11:32 AM
To: Geoff McAuliffe
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microglia antibody

Thanks for the suggestions.  I've used F4/80 in the past, I think this one was from serotec, but I used it in mouse sections.

Does anyone have experience with this antigen or antibody?

Thanks!


On 8/7/08 1:29 PM, "Geoff McAuliffe"  wrote:

> Hi Caroline:
> 
>     Both ED-1 and OX42 stain rat brain microglia, but different 
> populations. I will try to dig up a reference for you.
> 
> Geoff
> 
> Caroline Bass wrote:
>> Hello Everyone,
>> 
>> Iım looking for an antibody to stain microglia in rat brain sections.  
>> These will be perfused with formalin and sectioned at about 60 
>> microns.  Iıll probably start with an ABC kit, but would also like 
>> the ability to use the antibody for immunofluorescence. Are there any recommendations?
>> 
>> Thanks,
>> 
>> Caroline Bass
>> 
>> _______________________________________________
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>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> 
>> 
>>   
> 


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