RE: [Histonet] Microglia antibody

From:"Nancy Lemke"



Hi Caroline,
I use Serotec CD68 (MCA341R) with wonderful results.  I retrieve in citrate buffer pH 6.0 on a hotplate (boil 10 and cool 20), and dilute the antibody to 1:200, incubate for 30 minutes and use Biocare 4+ avidin-biotinHRP detection and DAB.  The stain is very clean and reliable.
Nancy Lemke

Nancy Lemke
Research Coordinator
Hermelin Brain Tumor Center
Henry Ford Hospital
Detroit
-----Original message-----
From: "Liz Chlipala" liz@premierlab.com
Date: Thu, 07 Aug 2008 13:25:09 -0400
To: "Caroline Bass" cbass@wfubmc.edu
Subject: RE: [Histonet] Microglia antibody

> Caroline
> 
> I believe you can use ED-1 or OX42 or both.  Both work in FFPE tissue > samples.
> 
> Liz 
> 
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Manager
> Premier Laboratory, LLC
> P.O. Box 18592
> Boulder, CO 80308
> phone (303) 682-3949
> fax (303) 682-9060
> liz@premierlab.com
> www.premierlab.com
>  
> Ship to Address:
>  
> Premier Laboratory, LLC
> 1567 Skyway Drive
> Unit E
> Longmont, CO 80504
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Caroline > Bass
> Sent: Thursday, August 07, 2008 11:13 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Microglia antibody
> 
> Hello Everyone,
> 
> Iım looking for an antibody to stain microglia in rat brain sections.  > These will be perfused with formalin and sectioned at about 60 microns.  > Iıll probably start with an ABC kit, but would also like the ability > to use the antibody for immunofluorescence. Are there any > recommendations?
> 
> Thanks,
> 
> Caroline Bass
> 
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