I am attempting to perform IHC-P on heavily pigmented melanocytic
growths using flourescent antibodies. I have tried using 10% H2O2
both overnite at room temperature and over two hours at 60 degrees.
The bleaching is decent but tissue integrity suffers greatly.
I have not tried the potassium permanganate/oxalic acid method yet
(although at this point I will have to try it) but I had a question
about the necessity of bleaching as I am not using chromagen-based
antibodies. The bleaching does not remove the pigment (correct?) so
it is not a question of primary antibody accessibility but of color
differentiation right? Since I am using flouresence microscopy is
bleaching the growths a necessary step?
Thanks for your time,
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