Yep, you don't have to bleach it because this is immunofloresece.
People bleach because melanin pigment can look a lot like the chromogen
DAB. I think it's better to just do it in red, if it were light microscopy
On 8/11/08, email@example.com wrote:
> I am attempting to perform IHC-P on heavily pigmented melanocytic growths
> using flourescent antibodies. I have tried using 10% H2O2 both overnite at
> room temperature and over two hours at 60 degrees. The bleaching is decent
> but tissue integrity suffers greatly.
> I have not tried the potassium permanganate/oxalic acid method yet
> (although at this point I will have to try it) but I had a question about
> the necessity of bleaching as I am not using chromagen-based antibodies.
> The bleaching does not remove the pigment (correct?) so it is not a
> question of primary antibody accessibility but of color differentiation
> right? Since I am using flouresence microscopy is bleaching the growths a
> necessary step?
> Thanks for your time,
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