You are trying to demonstrate Nissl substance?
Add 0.4 ml 10% acetic acid to 40 mls 0.1% cresyl echt violet in dist
water, warm (35 to 40 degrees C). Stain sections 6 min, differentiate in
95% alcohol, dehydrate in two changes of absolute alcohol, clear in
xylene and mount in synthetic resin.
DD 01934 647057 or extension 3311
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