[Histonet] question about cresyl violet

From:"Dr. Catherine L. Ryan"

I am having a problem with my cresyl violet stain. I am using cresyl
violet acetate (1%) on 50 micron formalyn fixed rat brain cut with a
vibratome.  I am losing all stain at the final  Histo-prep stage.  In
brief my procedure is as follows: I start with a series of alcohols (70-
95-100-95-70-50)- dH2O- cresyl violet-70%-95% EtOH - 95%+acetic 
acid to 95%-100% EtOH - Histo-prep wash.  Any ideas or sugggestions for 
an improved protocol, would be appreciated.
Dr. Catherine L. Ryan
Department of Psychology
University of Prince Edward Island
Charlottetown, P.E.I., Canada, C1A 4P3
Ph# (902)566-0323/  FAX#(902)628-4359

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