Re: [Histonet] autostainers & TMA's

From:"Helen Fedor"

Dear Garry,  In our TMA Core Facility we use Charged slides, routinely
we are using the Capillary Gap slides from Fisher.
we dry all TMA slides vertically overnight at room temperature.
and they store them at -20.

We do have experience tissue loss with this method.


Helen L. Fedor B.S.
Johns Hopkins University
Pathology Department
600 N Wolfe St
Marburg Room 406
Baltimore MD 21287
Phone: 410 614-1660
Pager: 410 283-3419

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>>> Garry Ashton  8/12/2006 8:38 AM >>>
Dear histonetters
I have 2 topics up for discussion.
Firstly immunostainers. 
We are a small research lab and hence I'm interested in a machine that
is totally flexible with regards to running several antibodies with 2
different methods.
Ideally an "open system" that will  also dewax & retrieve if possible.
We've looked at dako and biogenix which are both open  systems and
ventana and vision biosytems.
Any comments on the above, or on any other systems available would be
I know this subject ha been covered several times on the histonet, but
an up to date view would be much appreciated.
Secondly has anybody had any problems with regards to TMA sections
falling off slides.
We use 1mm cores and only put about 100 in each block, which are then
cut and placed on APES coated slides.
Sometimes up to 30% of the core may come off.
I think next we'll try charged slides. Does anybody have any opinions
ideas on this
Many thanks in advance for any opinions.
Garry Ashton

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