Re: [Histonet] Grinding & polishing of resin sections.
In a message dated 8/16/2005 2:00:20 AM US Mountain Standard Time,
> After grinding &polishing using grinding papers and diamond paste of
> different sizes we find the sections contain lots of debris.What can I do to clean
> these resin sections before it is stained?
Can you completely remove the plastic from the sections before staining?
This would certainly remove the bulk of the contaminants, since they're getting
embedded in the sections during the polishing process.
It would be similar to the deparaffinization of paraffin sections.
You could probably use 2-methoxy ethyl acetate to remove the methacrylate
plastic. Then you would simply have the specimens remaining on the slide, with
no plastic Wash and rehydrate thoroughly after the "deacrylation," and proceed
See the following link:
Robert (Bob) Chiovetti, Ph.D.
The Microscope Works
Arizona's Microscopy Resource
132 North Elster Drive
Tucson, AZ 85710-3212 USA
Member, Arizona Small Business Association - ASBA
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