We charge $25 for a handling fee for all specimens that need to be sent to a reference lab for additional testing. The only thing that we do not charge for is consults requested by our pathologists. Second opinions requested by the physician or patient do, however. 

Tina Mrosla
St. Joseph's Hospital
St. Paul, MN

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
histonet-request@lists.utsouthwestern.edu
Sent: Monday, August 15, 2005 12:27 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 21, Issue 19


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Today's Topics:

   1. mouse heart (adrienn kiss)
   2. Nikon relay lens (nyilmaz@mersin.edu.tr)
   3. CAP Info Needed (Heather.A.Harper@pcola.med.navy.mil)
   4. immuno peroxidase and autoradiography (Karen Fox-Talbot)
   5. Breast Implant Storage (Kopczynski, Charlotte)
   6. Shandon Citadel 2000 for sale (Denise Van Eaton)
   7. RE: Breast Implant Storage (Laurie Colbert)
   8. Re: Nikon relay lens (RCHIOVETTI@aol.com)
   9. handling fee specimens/tests sent to ref. lab
      (histology@gradymem.org)


----------------------------------------------------------------------

Message: 1
Date: Sun, 14 Aug 2005 11:58:16 -0700 (PDT)
From: adrienn kiss 
Subject: [Histonet] mouse heart
To: histonet@lists.utsouthwestern.edu
Message-ID: <20050814185816.68933.qmail@web54101.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hi,
I am new on the list and also in mouse heart
histology. I am wondering what is the best way to take
a heart for good histology. Until in situ, how long
the heart can be left without fixation before any
signs of necrosis would appear by histology? I mean in
a dead animal how long it can be left cyanotic before
major effects on morphology of the myofibers, cell
size, or immunohistochemistry to detect apoptosis,
etc. I would normally take the heart asap, however, it
is not possible always. Would perfusion with fixative
always be suggested? Is perfusion of the aorta with
fixative also suggested? Would retrograde perfusion
damage the enothelium?
Any help is appreciated,
Thanks a lot,
Adrienn Kis,
Postdoctoral research fellow
univeristy of Cambridge, England,



		
____________________________________________________
Start your day with Yahoo! - make it your home page 
http://www.yahoo.com/r/hs 
 



------------------------------

Message: 2
Date: Mon, 15 Aug 2005 09:33:48 +0300
From: 
Subject: [Histonet] Nikon relay lens
To: 
Message-ID: <20050815063348.6D9AA45587@mail.mersin.edu.tr>
Content-Type: text/plain;	charset="ISO-8859-9"

Hi All...

We're using an Olympus BX 50 light microscope attached with Nikon Coolpix 5000 digital camera. I'm wondering what the mangnification value of MDC relay lens between the camera and the microscope is. I could'nt find any information from internet or other sources. We can't estimate the final magnification value of pictures correctly without knowing that.
Thanks for the help.

Dr. Necat YILMAZ
Mersin University Medical School
Histology & Embryology Dep.
Mersin/TURKEY








------------------------------

Message: 3
Date: Mon, 15 Aug 2005 06:48:36 -0500
From: Heather.A.Harper@pcola.med.navy.mil
Subject: [Histonet] CAP Info Needed
To: histonet@lists.utsouthwestern.edu
Message-ID:
	<807FE48C5A7CC940B973B58D32E701431A850CCC@nhpens-exch1.pcola.med.navy.mil>
	
Content-Type: text/plain

Our hospital did the histology CAP, where you send in the requested slides,
and you can earn CE points by doing the final critique. I want and need to
know, when they send you back your results, is there any forms you have to
fill out like what corrective action was taken etc.. Since this is something
new that CAP has started, we are not sure what our next move is and I have
already received the next CAP packet and must have slides submitted no later
than Sept. 2 Also to get credit for the Critique, do I need to create a
password and log onto the CAP website? Any info is very much appreciated.

 

Heather A. Harper          

Histology Supervisor

Naval Hospital of Pensacola, FL

 



------------------------------

Message: 4
Date: Mon, 15 Aug 2005 08:30:41 -0400
From: "Karen Fox-Talbot" 
Subject: [Histonet] immuno peroxidase and autoradiography
To: 
Message-ID: 
Content-Type: text/plain; charset=US-ASCII

I would like to do  a combination stain of 
immunperoxidase and autoradiography (using Indium).  Does anyone have any experience with this?

Karen Fox-Talbot
Johns Hopkins University 
Department of Pathology

410-614-0915





------------------------------

Message: 5
Date: Mon, 15 Aug 2005 10:14:35 -0400
From: "Kopczynski, Charlotte" 
Subject: [Histonet] Breast Implant Storage
To: "'histonet@lists.utsouthwestern.edu'"
	
Message-ID:
	<5452D66669CFC540B0452115ED26AF1F0191AEFC@bcexch02.bcad.baycare.org>

I'm interested in how long you are keeping breast implants that have been
removed.  I have seen the recommendations on the CAP site but they do not
address the length of time these specimens should be kept.  I am trying to
get my Risk management Department to make a decision.  

Thanks,
Charlotte Kopczynski,HTL(ASCP)
Baycare Pathology Manager
Phone: 727-461-8246
Fax:   727-462-7597

Confidential:  This electronic message and all contents contain information
from BayCare Health System which may be privileged, confidential or otherwise
protected from disclosure.  The information is intended to be for the addressee
only.  If you are not the addressee, any disclosure, copy, distribution or use
of the contents of this message is prohibited.  If you have received this
electronic message in error, please notify the sender and destroy the original
message and all copies.



------------------------------

Message: 6
Date: Mon, 15 Aug 2005 09:56:21 -0500
From: "Denise Van Eaton" 
Subject: [Histonet] Shandon Citadel 2000 for sale
To: histonet@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; format=flowed

We have 3, due to some reinstrumentation. Ages are 1983, 1993,and 1996. All 
three are the
2000 model, no vacuum.Two of the three are in good working order, the third 
is missing a paraffin bath (cracked). Let us know, we'd sure like to find=20
them all a new home! thanks,denise

_________________________________________________________________
Express yourself instantly with MSN Messenger! Download today - it's FREE! 
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------------------------------

Message: 7
Date: Mon, 15 Aug 2005 08:00:24 -0700
From: "Laurie Colbert" 
Subject: RE: [Histonet] Breast Implant Storage
To: "Kopczynski, Charlotte" ,
	"Histonet (E-mail)" 
Message-ID:
	<0BE6ADFAE4E7E04496BF21ABD346628005653744@EXCHANGE1.huntingtonhospital=2Ecom>
	
Content-Type: text/plain;	charset="iso-8859-1"

Six months - the same as hardware.  We used to keep them indefinitely, but you can imagine what a mess that was.

Laurie Colbert

-----Original Message-----
From: Kopczynski, Charlotte [mailto:Charlotte.Kopczynski@baycare.org]
Sent: Monday, August 15, 2005 7:15 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Breast Implant Storage


I'm interested in how long you are keeping breast implants that have been
removed.  I have seen the recommendations on the CAP site but they do not
address the length of time these specimens should be kept.  I am trying to
get my Risk management Department to make a decision.  

Thanks,
Charlotte Kopczynski,HTL(ASCP)
Baycare Pathology Manager
Phone: 727-461-8246
Fax:   727-462-7597

Confidential:  This electronic message and all contents contain information
from BayCare Health System which may be privileged, confidential or otherwise
protected from disclosure.  The information is intended to be for the addressee
only.  If you are not the addressee, any disclosure, copy, distribution or use
of the contents of this message is prohibited.  If you have received this
electronic message in error, please notify the sender and destroy the original
message and all copies.

_______________________________________________
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------------------------------

Message: 8
Date: Mon, 15 Aug 2005 11:38:55 EDT
From: RCHIOVETTI@aol.com
Subject: Re: [Histonet] Nikon relay lens
To: nyilmaz@mersin.edu.tr, histonet@lists.utsouthwestern.edu
Message-ID: <1c3.2eb8ab83.3032110f@aol.com>
Content-Type: text/plain; charset="US-ASCII"

In a message dated 8/14/2005 11:35:35 PM US Mountain Standard Time, 
nyilmaz@mersin.edu.tr writes:

> We're using an Olympus BX 50 light microscope attached with Nikon Coolpix 
> 5000 digital camera. I'm wondering what the mangnification value of MDC relay 
> lens between the camera and the microscope is.


Dr. Yilmaz,

I recently set up a system on a Leica microscope, using a Nikon Coolpix 8800. 
 We did the following:

1.  We obtained a stage micrometer, which is a precise ruling that is etched 
on a microscope slide.  The one we used was 1 mm overall length, with smallest 
rulings of 0.010 mm (10 micrometers).

2. We did not use the MDC relay lens, but we purchased an adapter from 
another company.

3.  On our system, we had to use the optical zoom of the camera to obtain a 
full field of view (eliminate the vignetting, or "clipping" of the image at the 
corners).

4.  Since the zoom on the camera is infinitely adjustable, we had to decide 
on a reproducible zoom setting that always gave the same magnification.  For 
the Coolpix 8800 and our adapter, we opted to zoom to the maximum setting=2E  It's 
10x on the 8800; I'm not sure what it is on the 5000.

5.  To make measurements we set the camera to this maximum (10x) zoom, and 
took pictures of the stage micrometer with each objective.  From there, it's 
relatively easy to measure the spacing of the 10 micrometer lines and to 
calculate a total magnification.

This was the only way we could come up with to make measurements, since the 
camera's zoom is so variable.  It's a small inconvenience, but the camera works 
great with this set-up.

You could probably use a similar procedure, provided you are using the 
camera's optical zoom to fill the field of view with the MDC relay lens.  The trick 
is to use a zoom setting that is reproducible.

Hope this is of some help.

Best regards,

Robert (Bob) Chiovetti, Ph.D.
The Microscope Works
Arizona's Microscopy Resource
132 North Elster Drive
Tucson, AZ 85710-3212 USA
Tel./Fax 520-546-4986
Member, Arizona Small Business Association - ASBA


------------------------------

Message: 9
Date: Mon, 15 Aug 2005 10:50:19 -0500
From: histology@gradymem.org
Subject: [Histonet] handling fee specimens/tests sent to ref. lab
To: Histonet 
Message-ID: <2b50526ed7.26ed72b505@onenet.net>
Content-Type: text/plain; charset=us-ascii


Does anyone charge the patient a handling fee for specimens sent to a 
reference lab?  We send our cytologies out and used to charge a 
handling fee.  Any comments?

Angie 



------------------------------

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End of Histonet Digest, Vol 21, Issue 19
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