[Histonet] RE: Endogenous Peroxidase
Hello James and others,
Despite of all the (useless) chemical stories you've received so far I
think that there's mix-up of Na3N and NaN3 here. "Sodium nitride"
should be "sodium azide" = NaN3. That would fit in very well with your
subject line: "endogenous peroxidase". The mixture of PBS (or TBS)
with Na-azide and peroxide is a well known procedure to quench
endogenous peroxidase activity (Li et al., JHC 35:1457-1460, 1987).
The only thing that doesn't fit here is the fact it isn't new...
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
NL-1105 AZ Amsterdam
Date: Tue, 16 Aug 2005 07:28:36 +0000
From: "Mr James Hugh Reilly"
Subject: [Histonet] Endogenous Peroxidase
I have been asked to try a new Immumohistochemistry (IHC) technique
which uses 1% hydrogen peroxide and 2% sodium nitride in phosphate
buffer solution. Does anyone know what sodium nitride Is? and where
can it be purchased?
University Dept. Medicine
Level 3 QEB
10 Alexandra Parade
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