[Histonet] Embedding human cartilage/joint tissue in plastic

From:"Casey, Jane"


We are trying to use histology to validate our method of measuring cartilage thickness on wrist bones using ?CT scans. Initially we tried fixing a trapezoid fragment in formalin, de-mineralizing it, and embedding it in paraffin before slicing it into 5 ?m sections. This method led to non-uniform shrinkage of the tissue and clearly visible artifact. We would like to try a method, perhaps embedment in plastic, which will yield a more accurate measure of cartilage thickness. Does anyone have any suggestions?  
Thank you for your suggestions.
Jane Casey
Department of Orthopaedics 
Brown Medical School / Rhode Island Hospital
Providence, RI 02912

Histonet mailing list

<< Previous Message | Next Message >>