Re: [Histonet] Cutting fresh "squishy" rat brain

From:Gayle Callis

What about holding brain in one of the rat brain matrices holders.  It 
allows holding the brain for precise slices, coronal 
orientation. has these, for coronal, sagittal, and 
different age and size of rats.  You can cut as thin as 1 mm

At 10:38 AM 8/17/2004, you wrote:
>Hello everyone,
>I need to cut sections of fresh rat brain, in certain stereotactic 
>coordinates and then embed them in OCT.  Does anyone have any tips as to 
>how to do this without squashing the brain?  We are trying to cut coronal 
>sections of the brain to precisely expose a particular region, and we are 
>using a fresh disposable microtome blade to do the cuts, but the brain is 
>still very squishy when fresh and is very difficult to cut.   We are doing 
>LCM on these cryosections, so any type of fixation is out of the question.
>Kim Merriam
>Cambridge, MA
>Kim Merriam
>Cambridge, MA
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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