Re: [Histonet] Cutting fresh "squishy" rat brain
Thanks so much for this information about brain matrices from myneurolab and ted pella. The histonet can be a lifesaver!
Gayle Callis wrote:
What about holding brain in one of the rat brain matrices holders. It
allows holding the brain for precise slices, coronal
orientation. www.Myneurolab.com has these, for coronal, sagittal, and
different age and size of rats. You can cut as thin as 1 mm
At 10:38 AM 8/17/2004, you wrote:
>I need to cut sections of fresh rat brain, in certain stereotactic
>coordinates and then embed them in OCT. Does anyone have any tips as to
>how to do this without squashing the brain? We are trying to cut coronal
>sections of the brain to precisely expose a particular region, and we are
>using a fresh disposable microtome blade to do the cuts, but the brain is
>still very squishy when fresh and is very difficult to cut. We are doing
>LCM on these cryosections, so any type of fixation is out of the question.
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