RE: [Histonet] frozen tissue

From:"GUTIERREZ, JUAN" 
Yes, and it doesn't work.  That's why we use transport media.  The only time we got it to work was when the tissue had been in formalin for only a few minutes.  Good luck!
 
Juan

	-----Original Message----- 
	From: Martha Ward [mailto:mward@wfubmc.edu] 
	Sent: Fri 8/22/2003 7:58 AM 
	To: histonet@pathology.swmed.edu 
	Cc: 
	Subject: [Histonet] frozen tissue
	
	

	I have a situation that I hope I can get some help with.  My neuropahtologist had some nerve tissue in formalin for several weeks.  He gave the tissue to a resident, had him bisect the tissue and asked him to order immunofluorescent studies (IgG, IgM).  He told the resident to wash the tissue in water and OCT embedding media, freeze and cut sections.  Has anyone ever tried this and if so how did things turn out?  Thanks in advance for the help. 
	
	Martha Ward
	Wake Forest University Baptist Medical Center
	
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