RE: [Histonet] Borg Decloker, pH 9.5 and options.
I'm not sure which lab you work in, but a popular option may be our Trilogy
solution. This EDTA based solution is used by many labs as a universal
solution to unmask all their antibodies. It gives high-pH quality
unmasking for tougher antibodies like Cyclin D1, ALK-1, TdT, etc....but it isn't
high pH so it is less harsh on the tissue than some higher pH solutions.
We run all of the antibodies that we offer (with the exception of adenovirus and
Her2) with Trilogy antigen retrieval and get optimal staining
consistently. Trilogy allows for deparaffinization within the solution
while doing HIER, but it doesn't have to be used that way if that is not
preferred. It can be used simply as an epitope retrieval solution, just
like you would use regular citrate solution. And with no expiration
date, no refrigeration, and no diluting needed, it is a very efficient
reagent. Add to that that when you purchase the 5 gallon volume (sent in 5
one gallon jugs) it costs less than $80 A GALLON, making it one of, if not THE,
most economical reagents on the market. It works in clinical and research
settings and has been published in several papers, including from such respected
companies as Genentech. We invite you to evaluate it for your IHC
needs. If you would like to try it, please e-mail me and I will send you a
free sample so that you can see for yourself.
if you want to optimize your staining for some nuclear staining antigens and
some antigens that are very sensitive to fixation, like CD10, CD4, CD5, Cyclin
D1, TdT, TTF-1, ER, PR, Inhibin, WT1, etc., you many want to consider switching
to a pressure cooker, whose high heat tends to really bring out the tougher
epitopes to retrieve. We have found that it is fast (15 minutes with no
cooldown necessary) and consistent (since it is a closed system with constant
120 degrees centigrade temperature throughout heat retrieval). We have
many clients that use Trilogy in the pressure cooker (and many that use it in
the steamer as well) for all of their antibodies, even the ones they don't
purchase from Cell Marque. If you have any questions, please contact me
via e-mail or via phone at 1-800-665-7284, Ext. 12.
Director of Technical Sales and Marketing
I am trying to find a way to make or to procure from someone else a
buffer for antigen retreval, we steam.
Thanks for your help:
A. Kevin Williams
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