more info
I'm sorry about not giving enough information. I have had several
responses asking for more details. So, I'll try again!
I am performing IHC staining. I am using a DAKO autostainer with
the LSAB detection system. I use DAB for my chromagen. My negative
mouse and negative rabbit serum slides have quite specific staining
in the granlocytic series of WBC's(No matter what pretreatment -
I apply no pretreatment, Proteinase K, or use Microwave at pH 6 and
10). Microwave pretreatment is the worst, but I get the staining
most of the time. Polysegmented neutrophils seem to be the main
culprit. Most of the times my Docs can read through this, but they
are requesting my experimentation to find a "cure" for the situation,
so they can honestly say, "no staining was seen in the negative
control". One of the suggestions I have received is to do an Avidin/Biotin
Block. I will give this a try, but if there are any other ideas out
there: DON'T hold back! THANKS AGAIN!
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