RE: bkg IHC

From:"Favara, Cynthia (NIH/NIAID)"

Did I miss something?

What primary?

What controls?

Cynthia Favara
903 South 4th Street
Hamilton, MT 59840

-----Original Message-----
From: becky riesen []
Sent: Thursday, July 31, 2003 8:45 AM
Subject: bkg IHC

I have specific staining in polys on my negative controls when the 
section is a bone core from bone marrow biopsies.  All other specimen 
types seem to be fine.  I have doubled the time in my H2O2 to 12 
minutes.  I have purchased new H2O2 in case I had an old bottle. 
I'm increasing the time more, but I use an automated stainer and 
this will lengthen the time of my entire run and I'm concerned about 
getting too long here.  I wonder if there are other suggestions, 
solutions, procedures that you folks could share that may help my 
situation.  THANKS!

Naples Free-Net Web Mail

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