RE: Gallyas Stain on Brain

From:"Featherstone, Annette"

he Gallyas Stain presents some different problems because of the strength of
the periodic acid (5%). Do not use coated slides because the periodic acid
eats through the coating and leaves streaks all over the slides. It is
extremely important that the mouse tissue is processed very well, if it has
a hard time staying on an H&E it will never make the Gallyas.Try drying your
slides in a 37 degree oven overnight and then a 56degree oven the next day
for an hour. We have had much success with this method of drying on brain
tissue. Good luck and let me know if you have any other questions. We do
this stain all the time and also work with mouse and rat brain.
Annette HT/MLT

-----Original Message-----
From: Judi Ford []
Sent: Monday, August 04, 2003 13:34
Subject: Gallyas Stain on Brain


I've just finished doing the Gallyas stain for tangles and have found that
no matter how gentle I am with the slides tissue falls off the slide during
the stain.  I thought about using + slides but heard that brain tissue and
plus slides don't work due to +/- charges working against each other.  Any
suggestions?  Also, what could I use as a control for tangles?  I'm working
with mouse tissue. 

Thanks for your help.

Judi Ford
Jackson Laboratory, ME

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