Re: coated slides Elmer's
Allen , Gayle et. al.,
Bob Richmond may remember a discussion on this subject a few years ago.
Elmer's, as stated by Carlos and Louise(sic), is a vinyl derived substance,
a polyvinyl acetate. This adhesive is wholly synthetic, not a dairy or
animal product. It is not derived from a protein. A Google search can
confirm this. Is it sterile? Doubtful.
The Borden Company of today is something very different from what us geezers
remember with Elsie wearing a housedress and flowered apron. I doubt that
dairy products provide much of their current revenue. Borden is a major
industrial chemical manufacturing company among whose products is, believe
it or not, high purity formaldehyde, some of which is probably finding its
way into Histology labs.
----- Original Message -----
From: "Smith, Allen"
To: "louise renton"
Sent: Monday, August 04, 2003 10:58 AM
Subject: RE: coated slides Elmer's
Since Elmer's is made by Borden's (a dairy company), it is probably casein.
Allen A. Smith, Ph.D.
School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida 33161-6695
From: louise renton [mailto:firstname.lastname@example.org]
Sent: Monday, August 04, 2003 6:03 AM
Subject: Re: coated slides Elmer's
I have heard a lot about Elmer's Glue, which I gather is some type of
household vinyl based adhesive. We don't get this specific brand, but
similar ones, used specifically for woodwork or crafts are available.
So...my question is this: How is it used? Neat, Diluted, in the
waterbath??? PLease advise as I have some deadful whole paw sections that I
am having difficuly "sticking" to the slide.
BTW, I have tried chrome alum, but get horrible background so my thanks go
to Gayle for suggesting placing them in NBF. I will try that too.
Bone Research Unit
Tel & fax +27 11 717 2298
"Time flies like an arrow, fruit flies like a banana"
----At 08:48 a.m. 01/08/2003 -0400, you wrote:
>I am having problems with tissue staying on my slides after drying
>in 37C oven and then one more night in a 60C oven. The tissue is chicken
>leg knee joints. They are formalin fixed, EDTA decaled, and paraffin
>embedded samples. I think that coated slides may help. Does anyone have a
>simple protocol for making coated slides to help this tissue stick?
>Thanks in advance for the help.
>University of Rochester
You can use a vinyl glue like Elmer#180#s, it works fine in cases
like you are describiyng. The adhesion properties are at least
the same that charged slides when you are treating samples
like cartilage and bone.
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