Re: coated slides Elmer's

From:"Mark Ray"

Allen , Gayle et. al.,

Bob Richmond may remember a discussion on this subject a few years ago.
Elmer's, as stated by Carlos and Louise(sic), is a vinyl derived substance,
a polyvinyl acetate.  This adhesive is wholly synthetic, not a dairy or
animal product. It is not derived from a protein.  A Google search can
confirm this.  Is it sterile?  Doubtful.

The Borden Company of today is something very different from what us geezers
remember with Elsie wearing a housedress and flowered apron.  I doubt that
dairy products provide much of their current revenue.  Borden is a major
industrial chemical manufacturing company among whose products is, believe
it or not, high purity formaldehyde, some of which is probably finding its
way into Histology labs.

Mark Ray

----- Original Message ----- 
From: "Smith, Allen" 
To: "louise renton" 
Cc: 
Sent: Monday, August 04, 2003 10:58 AM
Subject: RE: coated slides Elmer's


Since Elmer's is made by Borden's (a dairy company), it is probably casein.

Allen A. Smith, Ph.D.
Barry University
School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161-6695

-----Original Message-----
From: louise renton [mailto:louise_renton@hotmail.com]
Sent: Monday, August 04, 2003 6:03 AM
To: histonet@pathology.swmed.edu
Subject: Re: coated slides Elmer's


Dear all

I have heard a lot about Elmer's Glue, which I gather is some type of
household vinyl based adhesive. We don't get this specific brand, but
similar ones,  used specifically for woodwork or crafts are available.
So...my question is this: How is it used?  Neat, Diluted, in the
waterbath???  PLease advise as I have some deadful whole paw sections that I

am having difficuly "sticking" to the slide.

BTW, I have tried chrome alum, but get horrible background so my thanks go
to Gayle for suggesting placing them in NBF. I will try that too.

Best regards



Louise Renton
Bone Research Unit
MRC
Johannesburg
South Africa
Tel & fax +27 11 717 2298
"Time flies like an arrow, fruit flies like a banana"





----At 08:48 a.m. 01/08/2003 -0400, you wrote:
>Hi,
>I am having problems with tissue staying on my slides after drying
>overnight
>in 37C oven and then one more night in a 60C oven.  The tissue is chicken
>leg knee joints.   They are formalin fixed, EDTA decaled, and paraffin
>embedded samples.  I think that coated slides may help.  Does anyone have a
>simple protocol for making coated slides to help this tissue stick?
>Thanks in advance for the help.
>
>Loralee Gehan
>University of Rochester

>   Loralle:
You  can  use  a  vinyl glue  like  Elmer#180#s, it  works  fine  in  cases
like you  are  describiyng. The  adhesion  properties  are  at  least
the  same  that  charged slides  when  you are  treating  samples
like  cartilage  and bone.
Good luck,Carlos.

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