Re:problem with in situ hybr.
Beste
Aniko,
There may be two reasons for the precipitations in your stainings.
First it may be that you have precipitation of your anti-dig antibodies.
The precipitates are small and without a seemingly identical structure
(like dirt particles). This can be solved by centrifuge your diluted
anti-dig antibody solution at high speed (around 10000 RPM). The second
reason may be christalline structures (like needles), which we also have,
if we use fast blue, dry the sections and embed in entellan. This, you
should not have if you embed with a watersoluble embedding
medium.
groetjes uit Wenen,
Jan
-----------------------------------------------------
Jan Bauer, Ph.D
Div. of Neuroimmunology
Brain Research Institute
Spitalgasse 4
A-1090 Vienna
Austria
tel: +43-1-4277 62813
fax: +43-1-4277 9628
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