Re:problem with in situ hybr.

From:Jan Bauer

Beste Aniko,

There may be two reasons for the precipitations in your stainings.
First it may be that you have precipitation of your anti-dig antibodies. The precipitates are small and without a seemingly identical structure (like dirt particles). This can be solved by centrifuge your diluted anti-dig antibody solution at high speed (around 10000 RPM). The second reason may be christalline structures (like needles), which we also have, if we use fast blue, dry the sections and embed in entellan. This, you should not have if you embed with a watersoluble embedding medium.

groetjes uit Wenen,

Jan Bauer, Ph.D
Div. of Neuroimmunology
Brain Research Institute
Spitalgasse 4
A-1090 Vienna

tel:    +43-1-4277 62813
fax:    +43-1-4277 9628

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