Nafiesa,
I am still struggling with my COX-2 staining as well; but you might try 
incubating your primary antibody overnight at 4 C.  Good luck.


Date sent:      	Mon, 19 Aug 2002 08:49:27 +0200
From:           	"Allie, N, Nafiesa," 
Subject:        	Cox-2 antibody
To:             	histonet@pathology.swmed.edu

> Hi Everyone
> I'm hoping that somebody will have some advise about the Cox-2 
> antibody from BD Transduction Laboratories, Cat#  610203.
> I've tried antigen retrieval with Citrate Buffer, diluting the 
> antibody at 1:100, incubating for 30min, and using Envision(Dako), 
> with no success. If anyone can give some advise, it will be very 
> appreciated.
> Thank you..
> Nafiesa
> #004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004#
> Nafiesa Allie
> Dept. Anat.Path.
> Medical School
> Univ.of Cape Town
> Anzio Rd.
> Observatory
> 7925
> Rep. of South Africa
> #004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004##004#
> 


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