Immunofluorescence or histochemistry methods for mitochondrial stain
Hallo everybody,
I have some frozen sections and
4% paraformaldehyde fixed/paraffin embedded sections of respiratory epithelium I
would like to stain for mitochondria. I'm trying some immunofluorescence
methods, but looking for suggestions in HistoNet I found out that there are also
some histochemistry methods to visualize mitochondria in tissues (Cain's,
Novelli...).
Since I'm not an experienced histologist, can
anybody give me some advices/references (where maybe I could
find a detailed protocol) about these methods?
Thank you very much in advance!
Alessandra
Dr. Alessandra Livraghi, PhD
UNC School of
Medicine
CF/Pulmonary Research and Treatment Center
CB#7248 7013
Thurston-Bowles Bldg.
Chapel Hill NC 27599
tel 919-966-7045
fax
919-966-7524
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