|From:||"Smith, Allen" |
Allen A. Smith, Ph.D.
School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida 33161-6695
From: Alessandra Livraghi [mailto:firstname.lastname@example.org]
Sent: Tuesday, August 27, 2002 5:03 PM
To: HistoNet Server
Subject: Immunofluorescence or histochemistry methods for mitochondrial stainHallo everybody,I have some frozen sections and 4% paraformaldehyde fixed/paraffin embedded sections of respiratory epithelium I would like to stain for mitochondria. I'm trying some immunofluorescence methods, but looking for suggestions in HistoNet I found out that there are also some histochemistry methods to visualize mitochondria in tissues (Cain's, Novelli...).Since I'm not an experienced histologist, can anybody give me some advices/references (where maybe I could find a detailed protocol) about these methods?Thank you very much in advance!AlessandraDr. Alessandra Livraghi, PhD
UNC School of Medicine
CF/Pulmonary Research and Treatment Center
CB#7248 7013 Thurston-Bowles Bldg.
Chapel Hill NC 27599