RE: Immunofluorescence or histochemistry methods for mitochondrial stain

From:"Smith, Allen"

Mitochondria can be displayed in frozen sections by showing their succinate dehydrogenase activity.
The reference is: Nachlas, M.M. et al (1957) Cytochemical demonstration of succinic dehydrogenase by the use of a new
p-nitrophenyl substituted ditetrazole.  J. Histochem. Cytochem. 5: 420-436.
Their method is summarized in many lab manuals, e.g. Kiernan, J. (1990) Histological and Histochemical Methods, 2nd ed., p. 270.
(My copy of the third edition is downstairs in my lab, and I'm too lazy to go look for the page number in it!)

Allen A. Smith, Ph.D.
Barry University
School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161-6695

-----Original Message-----
From: Alessandra Livraghi []
Sent: Tuesday, August 27, 2002 5:03 PM
To: HistoNet Server
Subject: Immunofluorescence or histochemistry methods for mitochondrial stain

Hallo everybody,
    I have some frozen sections and 4% paraformaldehyde fixed/paraffin embedded sections of respiratory epithelium I would like to stain for mitochondria. I'm trying some immunofluorescence methods, but looking for suggestions in HistoNet I found out that there are also some histochemistry methods to visualize mitochondria in tissues (Cain's, Novelli...).
Since I'm not an experienced histologist, can anybody give me some advices/references (where maybe I could find a detailed protocol) about these methods?
Thank you very much in advance!
Dr. Alessandra Livraghi, PhD
UNC School of Medicine
CF/Pulmonary Research and Treatment Center
CB#7248 7013 Thurston-Bowles Bldg.
Chapel Hill NC 27599
tel 919-966-7045
fax 919-966-7524

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