I'd also be interested in this staining procedure for a current project 
that I am working on using mouse aortas.
Andi

>Hi,
>
>Does anyone have an ORO staining protocol on non embedded mouse aorta? In 
>the procedure the mice hearts are perfused with a desired fixative for a 
>determined amount of time. Then the aorta is dissected out and stained 
>with ORO while the aorta is intact. After staining, the aorta is then 
>bisected and photographed. No processing or embedding is used. I have 
>found several abstracts but most are vague about the staining procedure. 
>Has anyone had an experience with this technique?
>
>    I believe I told a histonetter that I would forward a Movats protocol 
> to them but I lost the email... If this is still needed, please contact 
> me and I will send it.
>
>Betsy Molinari  HT  ASCP
>
>Texas Heart Institute
>
>Houston, TX 77373
>
>832-355-6524
>
>832-355-6812 (fax)

.....................................................................
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