Ag retrieval vs. Permeabilizing

From:Manju Kaushal

Saponin & triton both of them are detergents.
The function of these detergents is to increase the surface tension of
the cell membranes so the cells are more exposed than otherwise without
Does it make any difference - depends on your technique - sometime it
does, sometime it doesn't.

Manju Kaushal
Senior Manager
Immunopathology Service
Johns Hopkins Medical System
Weinberg Building - Rm 2323
401 North Broadway
Baltimore MD 21287
PH 410 614 6650
Fax 410 502 1732

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