Re: Automated tissue processing + propylene oxide

From:Violetta J Martinez

We used a lynx EM processor and epon/araldite mixture with 100% acetone as
our solvent for infiltration. It was quite sucessful. i beleive it is also
safer than Proplene oxide. Try it!
Violetta Martinez,MLT
Pathology, LHSC
London Ontario

Jamie Chapman wrote:

> G'day,
> Our lab is just about to get an EMS LYNX Automated Tissue Processor for
> processing tissues for routine transmission electron microscopy. One of
> the problems that our lab encountered with an older (and really quite
> massive automated machine - a Sakura - about the size of a Volkswagen)
> machine was that by the time the samples got to the propylene oxide
> intermediary step, the propylene oxide had evaporated.
> We are using Procure 812 as our resin. Does anyone know if the new
> machine has overcome this problem? If not, is it possible to use another
> processing protocol with Procure 812 that doesn't involve the propylene
> oxide step?
>  Hope you can help.
>  Cheers,
>  Jamie
>  Mr Jamie Chapman BSc(Hons)
>  Senior Technical Officer, Electron Microscopy
>  Department of Anatomical Pathology
>  Royal Hobart Hospital
>  Hobart, Tasmania 7001
>  ph. +61 3 6226 4837
>  fax +61 3 6226 4833

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