Roy, 

Thanks.  

The Carnoys I works as effectively as the Carnoys II.  Taking
toxic/carcinogenic choloroform out does not affect fixation, since it is
not a fixative anyway.  It is there to remove lipids from fatty tissues,
and for safety's sake, we prefer Carnoys I, and have had equivalent,
excellent results. 

  At 08:26 AM 8/7/02 +0930, you wrote:
>Atoska
>According to Lillie, Histologic Technic and Practical Histochemistry, 3rd
>edition 1965. Carnoy II is the alcohol, chloroform, acetic acid mixture that
>is generally referred to as Carnoy's fluid. Carnoy I is an alcohol, acetic
>acid mixture minus the chloroform.
>Regards
>Roy

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

email: gcallis@montana.edu