EM sample prep
I have been asked to prepare IHC-stained slides for EM.
What I was told to do (by the EM lab we are working with) is
to stain cryostat sections with my antibodies (a directly
biotinylated primary and immunogold secondary), then
post-fix them in glutaraldehyde/sodium cacodylate fixative
and send them to the EM lab for further processing.
Here are my questions: Do these samples need to be cryostat
or can I do this with paraffin? Wouldn't paraffin-embedded
samples have better morphology?
I have never done any type of EM, so I am clueless!
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