Re: Gomoris one step trichrome
Ian Clarke at Craigavon Area Hospital in North Ireland asks:
>>I am looking for the constituents of Gomori's one step trichrome. Is it
comparable to the Masson Trichrome Technique?<<
Comparable to the extent that it stains nuclei with hematoxylin, fibrous
tissue green, and parenchymal tissue red. But rather different from there.
What do you want to stain? Many people use it to stain frozen sections of
skeletal muscle. This Engel-Cunningham modification, usually not so credited,
was invented by W. King Engel (neurologist) and Guy Cunningham (histologist
and brains in the outfit) before 1968. It took me quite a while to get it
working when I was doing skeletal muscle histochemistry about thirty years
Wheatley's modification of the Gomori trichrome is widely used for staining
liquid stool smears for protozoan parasites. It has had many modifications in
recent years, partly because of the bewildering variety of intestinal
protozoan parasites that give HIV positive people intractable diarrhea.
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