We perform the one step Trichrome here and have for several years. We buy 
our kit from Poly Scientific with the blue counterstain. You can also buy 
it with the green counterstain. We find it to be quicker than the old 
method and the pathologists can't tell the difference.




At 04:33 PM 8/8/2001 -0600, Gayle Callis wrote:
>Victor Tobias put a one step Trichrome on Histonet a few years back if I
>recall correctly,  probably in the Archives.  Hopefully he is looking in.
>
>I think you can use either light green or aniline blue for connective
>staining component.
>
>At 09:39 PM 8/8/01 +0100, you wrote:
> >Hi all ,
> >I am looking for the constituents of Gomoris one step Trichrome,is it
> >comparable to the Masson Trichrome Technique?
> >Thanks in advance
> >Ian Clarke
> >Craigavon Area Hospital
> >N.Ireland
> >
> >
> >
> >
>Gayle Callis
>MT,HT,HTL(ASCP)
>Histopathology Supervisor
>Veterinary Molecular Biology
>Montana State University - Bozeman
>Bozeman MT 59717-3610
>
>406 994-6367
>406 994-4303 (FAX)


**************************************************************************
Jill Songer HT (ASCP)
Supervisor, Anatomic Patholog