We use DAKO's CK5/6 at 1:100 overnight (evidently, not a very potent antibody!) with waterbath HIER.  We use "tonsil" as our positive control.

R. Cartun

>>> THERESA ROHR <trohratnyackhospital@hotmail.com> 08/06/01 02:19PM >>>
Hi!

I am again seeking help with a "new" antibody for my lab. Is anyone using 
DAKO CK 5/6 currently with Envision Plus on the Autostainer?

If so, what dilution is working well for you?  Also, what tissue would you 
suggest as a good control?

It is great having access to your "minds" and also hearing from DAKO Reps. 
This site is saving me a lot of time and money by narrowing down my dilution 
testing both in terms of time and reagents.

Thanks in advance,
Theresa Rohr BA HT(ASCP)
Nyack Hospital
New York

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