sirius red for collagen

From:Gayle Callis <>


There is an excellent discussion in Histonet archives (John Kiernan and
others) on sirius red as a collagen stain.  Fast blue escapes me, but it
may be the counterstain for this purpose, my fast blue is used for alk phos
immunostaining protocols. This may be only a partial "------ fast blue" per
comments from others this morning.  

This is a lovely collagen stain, and viewed very nicely with polarized
light. I have some original articles stuffed into a file somewhere, if you
need them, they may be in the archives already.

Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-361

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