The edge effect comes more into play from the outer edges of the actual
specimen air drying, not so much from the section on the slide itself. 
 

David A. Reynolds, HT(ASCP) 
SNBL USA, Ltd. 
6605 Merrill Creek Parkway 
Everett, WA  98203 


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-----Original Message-----
From: JHoffpa464@aol.com [mailto:JHoffpa464@aol.com]
Sent: Wednesday, August 08, 2001 6:15 AM
To: histonet@pathology.swmed.edu
Subject: edge effects


OK a question for my own gratification. What causes the so called edge
effect 
i.e. increased none specific staining around the edge of sections, with IHC.

I have never seen the effect with IF. I know what everyone says, it's from 
partial drying around the edge of sections. However I have seen it even in 
sections completely flooded. 
John