I am trying to get good staining with these three antibodies on the DAKO 
autostainer using Envision +.  Has anybody gotten good results with these 
antibodies?  I need to know length of incubation of primary antibody and if it 
is at room temperature, 4 degrees C, or 37 degrees C; method of antigen 
retrieval, and control tissue used.  Thanks for any help. 

Bob Meyer, HTL 
Pathology Core Facility 
Nort