Presoaking sponges, an old trick

From:Gayle Callis <>

Good advice, Joyce!

This is an old trick and works for unfixed/partially fixed tissues going
into plastic cassettes.  Just dip tissue in fixative to rapidly fix a
miniscule layer of cells, and tissue should not stick to plastic.  

Too thick a tissue, or squishing the sponge against the tissue by jamming a
lid down is another thing.  I have seen lid marks on the tops of tissues
and sponge could do this to tiny biopsy if care is not taken to have
adequate space.  

Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610

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