Picro-Sirius Red for Collagen Quantification?
|From:||Tracey Couse <firstname.lastname@example.org>|
I am interested in a collagen stain that will allow one of our graduate
students to quantify the amount of total collagen present in coronary
artery sections. I have been reviewing the archives and literature for this
information. Based on my research thus far, picro-sirius red seems the
best stain for this purpose. I am not interested in the birefringence
properties of collagen and this stain as it is mainly collagen fiber (type
I only?) birefringence that is enhanced. Is picro-sirius the best stain to
use in determining the total collagen present in a given section using
ordinary bright field microscopy?
Any comments or suggestions would be greatly appreciated. Thanks in advance.
Petit Institute for Bioengineering & Bioscience
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