Hi again.  Thank you all so much for your prompt suggestions.
I forgot to mention that my control antibody is clean.  Very clean.
Could this imply a "dirty" primary?
I'm going to call the company and ask about adsorption with mouse IgG's.

>  -----Original Message-----
> From: 	Philopena, Jennifer  
> Sent:	Tuesday, August 14, 2001 11:57 AM
> To:	'Histonet' (E-mail)
> Subject:	background in FFPE spleen
> 
> Hi.  I'm desperate for suggestions.  Please HELP!
> I'm doing IHC on FFPE nude mouse spleen, and I'm having a BIG background
> issue.  I do antigen retrieval with citrate buffer, 3% peroxidase quench
> for 10 minutes, protein block - 30min, rabbit polyclonal antibody - 60min,
> biotin goat anti-rabbit secondary - 30min, SA-HRP - 15min, AEC-12min,
> hematoxylin.
> Any suggestions?
> Thanks a lot.
> Jennifer
> 
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