hucker/twort gram stain

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From:Kari Dalziel <>
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Dear histonetters,
   We've been doing the Hucker/Twort gram stain for a short time.  For some 
reason the fast green is very light.  The procedure that we have came from 
a histo school in the Midwest. For the fast green it just says fast green 
in ethanol.  So we've made it up with green to 100 ml. Reagent 
alcohol.  It's not staining all that well.  Does anyone out there have a 
more complete procedure?  It references Theory and Practice of Histological 
Technique, Bancroft and Stevens, 3rd Ed p 291.
Thanks in advance,
Kari Dalziel

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