I'm not sure that you need to destain.  I have done many immunos on H&E
stained slides.  I just exclude the counterstain.

Tom Mc Nemar
Pathology Supervisor
Licking Memorial Hospital
Newark, Ohio

> -----Original Message-----
> From:	Amy Self [SMTP:AmyS@gmhsc.com]
> Sent:	Wednesday, August 16, 2000 3:24 PM
> To:	'Histonet@Pathology.swmed.edu'
> Subject:	IHC on cytology specimen
> 
> 	
> 	I have to do a chromogranin on a FNA parotid mass slide that has
> already been PAP stained.  What is the best way to destain this slide?
> Also,
> I am just learning immuno's and thank God my lab bought a Nexes from
> ventana. Does anyone have any suggestions/procedure about this stain that
> will help me out?  I have only done immuno's on paraffin sections and this
> is the ONLY slide that we have on this case.  If anyone can help please
> e-mail privately or call me at 843-527-7179.
> 
> 					Thanks,
> 				                Amy