I have not tried freezing the matrigel, but we have implanted it in rats
and mice ( injected with a drug or tumor cells) later harvested it (wrapped
it in lense paper) and processed it in paraffin.
Gina
Gilead Sciences








                                                                                          
                    "Yandl, Emily"                                                        
                    <Emily.Yandl@ge        To:     "'histonet@pathology.swmed.edu'"       
                    nzyme.com>             <histonet@pathology.swmed.edu>                 
                                           cc:                                            
                    08/17/00 11:41         Subject:     freezing and sectioning Matrigel  
                    AM                                                                    
                                                                                          
                                                                                          



Hi everyone,

I'm wondering if anyone out there has experience working with a substance
called Matrigel?  An investigator here is trying to freeze blocks of this
gel with cells in 24-well plates and is experiencing difficulties with
morphology preservation (suprise!).  If you have worked with gel, I would
love advice on freezing and/or cryopreservation techniques.

Thanks,

Emily Ezernieks
Genzyme Corp.
emily.yandl@genzyme.com