Re: freezing and sectioning Matrigel

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To:"Yandl, Emily" <>
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I have not tried freezing the matrigel, but we have implanted it in rats
and mice ( injected with a drug or tumor cells) later harvested it (wrapped
it in lense paper) and processed it in paraffin.
Gilead Sciences

                    "Yandl, Emily"                                                        
                    <Emily.Yandl@ge        To:     "''"       
          >             <>                 
                    08/17/00 11:41         Subject:     freezing and sectioning Matrigel  

Hi everyone,

I'm wondering if anyone out there has experience working with a substance
called Matrigel?  An investigator here is trying to freeze blocks of this
gel with cells in 24-well plates and is experiencing difficulties with
morphology preservation (suprise!).  If you have worked with gel, I would
love advice on freezing and/or cryopreservation techniques.


Emily Ezernieks
Genzyme Corp.

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