Methacarn is a wonderful fixative for most IHC applications. For more
information on methcarn fixation please see the following articles:

Puchtler H, Waldrop FS, Meloan SN, Terry MS, Conner HM.
Methacarn (methanol-Carnoy) fixation. Practical and theoretical
considerations. Histochemie. 1970;21(2):97-116.

Mitchell D, Ibrahim S, Gusterson BA.
Improved immunohistochemical localization of tissue antigens using modified
methacarn fixation. J Histochem Cytochem. 1985 May;33(5):491-5.

McGinley JN, Knott KK, Thompson HJ.
Effect of fixation and epitope retrieval on BrdU indices in mammary
carcinomas. J Histochem Cytochem. 2000 Mar;48(3):355-62.


Regards,

John McGinley, HTL (ASCP)
AMC Cancer Research Center
Denver, CO