We've found the super sensitive alk phos from Biogenex kit to work well
here. No retreival.
Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta


	-----Original Message-----
	From:	Carla Shoffeitt [SMTP:histo92@hotmail.com]
	Sent:	Tuesday, August 22, 2000 12:00 PM
	To:	spectech@path.som.sunysb.edu; histonet@pathology.swmed.edu
	Subject:	Re: kappa and lambda IHC

	Michelle,
	  For our lab, we have found that Dako's Kappa and Lambda antibodies
work 
	best.  We use predilutes with pretreatment of Proteinase K (Dako).
We use 
	Dako's LSAB2 detection kit, as well as their autostainer.

	Carla Shoffeitt
	Columbus Regional Medical Center
	Columbus, GA.


	>From: Special Histology Lab Tech <spectech@path.som.sunysb.edu>
	>To: histonet@pathology.swmed.edu
	>Subject: kappa and lambda IHC
	>Date: Mon, 21 Aug 2000 14:08:30 -0400
	>
	>To All:
	>We have been having some problems with the consistency of staining
	>for  kappa and lambda on formalin fixed tissue. Just wondering if
we
	>can get some feedback on which abs. and what kind of pretreatments
	>are being used. Thanks.
	>
	>Michele Margiotta
	>Special Histology Lab
	>UH @ Stony Brook, NY
	>

	
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