RE: kappa and lambda IHC

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From:"Weems, Joyce" <>
To:'Carla Shoffeitt' <>,,
Content-Type:text/plain; charset=iso-8859-1

We've found the super sensitive alk phos from Biogenex kit to work well
here. No retreival.
Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta

	-----Original Message-----
	From:	Carla Shoffeitt []
	Sent:	Tuesday, August 22, 2000 12:00 PM
	Subject:	Re: kappa and lambda IHC

	  For our lab, we have found that Dako's Kappa and Lambda antibodies
	best.  We use predilutes with pretreatment of Proteinase K (Dako).
We use 
	Dako's LSAB2 detection kit, as well as their autostainer.

	Carla Shoffeitt
	Columbus Regional Medical Center
	Columbus, GA.

	>From: Special Histology Lab Tech <>
	>Subject: kappa and lambda IHC
	>Date: Mon, 21 Aug 2000 14:08:30 -0400
	>To All:
	>We have been having some problems with the consistency of staining
	>for  kappa and lambda on formalin fixed tissue. Just wondering if
	>can get some feedback on which abs. and what kind of pretreatments
	>are being used. Thanks.
	>Michele Margiotta
	>Special Histology Lab
	>UH @ Stony Brook, NY

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