DAB bleach reaction

<< Previous Message | Next Message >>
From:Gayle Callis <uvsgc@msu.oscs.montana.edu>
To:histonet@pathology.swmed.edu
Reply-To:
Content-Type:text/plain; charset=us-ascii

I don't think they are sure exactly what chlorinated hydrocarbons are
formed, but it is best not to do it and you CANNOT dump this  down the
drain.  IF it is unknown, the resulting chemicals from bleach treatment,
then you would not want to be downstream from this!  

Dick Dapson has a protocol using sulfuric acid/potassium permanganate
treatment that was supposed to destroy the DAB molecule properly, but you
still have to prepare and handle hazardous chemicals. I found this messy
and time consuming, but we now have chemical safety pick up used DAB and
haul it away to whereever dead chemicals are shipped.      

There are some great commercially sold DAB handling systems, DAB Out if I
recall which are very effective, and minimize you having to make up
sulfuric acid, permanganate solutions, and nicely remove or treat the DAB
correctly.  I think this is worth the investment.





Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303



<< Previous Message | Next Message >>