Hi All,

Just doing some risk assessments. A bit worried to see that 2g of sodium barbiturate is a human lethal dose. Only method we use that uses this buffer is Leders enzyme stain.

Could I substitute something safer than barbiturate buffer ?

(Having a good sort through our old chemicals  - aniline has joined todays list of redundancies !)

Richard Pitman FIBMS,
Head MLSO,
Dept of Histology, Cytology & Immunology,
Worcester Royal Infirmary NHS Trust