Karen,
I have an old (1970) copy of "Diagnostic Histochemistry" by Frederick 
T. Zugibe, with a protocol for Timm's Copper Stain,  pp. 254-55.

My experience with the stain tells me you can stain for all heavy 
metals (Zn, Fe, Hg, Cu, etc.) or select for Cu, but to selectively 
stain for zinc, goes beyond my handle on chemistr. Of course, that is 
not to say it can't be done!

If you want the method faxed to you let me know, I'd be happy to do 
so.  Greg 

> Date:          Tue, 27 Apr 1999 10:43:19 -0400 (EDT)
> From:          Karen Keller <klkeller@arches.uga.edu>
> Subject:       Re: Timm's stain protocol
> To:            HistoNet Server <HistoNet@Pathology.swmed.edu>

> 
> I'm interested in a protocol for a Timm's stain procedure for localizing
> zinc in the rat hippocampus.  Also, do any companies have a kit for this
> procedure?
> 
> Thanks,
> 
> Karen Keller
> Department of Physiology and Pharmacology
> College of Veterinary Medicine
> University of Georgia
> Athens, GA 30602
> 
> 
> 
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>
>
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>
>
Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
550 Unviversity Ave.
Charlottetown, P.E.I.
C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851