Dear Histonetters,

    I will soon be working on a double staining IHC project. I seem 
to remember someone discussing a proceedure that involved stripping 
off the first primary, after addition of the chromogen, and then 
applying the second primary. Does anyone have this protocol or any 
others involving double staining. One project involves two rabbit 
primary antibodies and there is not enough antibody to conjugate 
either one to HRPO or alk-phos. Thanks to all in advance. 

Cindy Chard-Bergstrom, BS, HT(ASCP)